Type

Journal Article

Authors

Paula Meleady
Annemarie Larkin
Martin Clynes
Michael Henry
Paul Moriarty
Stephen Beatty
Deirdre O'Flynn
Susan Kennedy
Annett Linge

Subjects

Biochemistry

Topics
uveal neoplasms female genetics diagnosis metabolism proteomics melanoma aged male biosynthesis tumor markers biological eye proteins gene expression regulation neoplastic blotting western middle aged pilot projects immunohistochemistry tumor cells cultured follow up studies secondary two dimensional difference gel electrophoresis polymerase chain reaction adult rna neoplasm humans

Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not. (2012)

Abstract To compare the proteomic profiles of two categories of primary uveal melanoma tissue samples; those from patients who have subsequently developed metastatic disease and those who have not. Two-dimensional difference gel electrophoresis (2D DIGE) was performed on 25 uveal melanoma tissue specimens (minimum follow-up of 7 years) comparing nine uveal melanoma tumors from patients who developed metastatic disease and 16 from those who did not. Most of the tumors which metastasized also exhibited chromosome 3 monosomy. Selected differentially expressed proteins were further followed up by immunohistochemistry and functional validation in vitro using siRNA. Proteomic analysis revealed 14 statistically significant differentially expressed proteins, with nine showing increased expression (PDIA3, VIM/HEXA, SELENBP1, ENO1, CAPZA1, ERP29, TPI1, PARK7, and FABP3) and five showing decreased expression (EIF2S, PSMA3, RPSA, TUBB, and TUBA1B) in uveal melanomas that subsequently metastasized compared with those that did not. Immunohistochemical analysis was performed for six of the differentially expressed proteins and gave similar results to the 2D DIGE study for two of these proteins, fatty acid-binding protein, heart-type (FABP3) and triosephosphate isomerase (TPI1). siRNA knockdown in the 92.1 uveal melanoma cell line confirmed a functional role for FABP3 and TPI1 in invasion in vitro. Proteomic analysis identified proteins differentially expressed in uveal melanoma that will subsequently metastasize, some of which appear to have a functional role in invasion. These results may contribute to better predictive tests (along with genetic analysis) and to the identification of new therapeutic targets.
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Full list of authors on original publication

Paula Meleady, Annemarie Larkin, Martin Clynes, Michael Henry, Paul Moriarty, Stephen Beatty, Deirdre O'Flynn, Susan Kennedy, Annett Linge

Experts in our system

1
Paula Meleady
Dublin City University
Total Publications: 95
 
2
Annemarie Larkin
Dublin City University
Total Publications: 25
 
3
Martin Clynes
Dublin City University
Total Publications: 209
 
4
Michael Henry
Dublin City University
Total Publications: 78
 
5
Susan Kennedy
Dublin City University
Total Publications: 37