Type

Journal Article

Authors

Christine E Loscher
Richard O'Kennedy
Edwina Stack
Johanna C Bruneau

Subjects

Biochemistry

Topics
macrophages cytokines metabolism immunosuppressive agents toxicity animals drug effects toll like receptor 4 toll like receptor 2 aflatoxins tlr2 protein mouse cell survival carcinogens cell line antigens cd14 mice tlr4 protein mouse

Aflatoxins B(1), B(2) and G(1) modulate cytokine secretion and cell surface marker expression in J774A.1 murine macrophages. (2011)

Abstract Aflatoxins are fungal products which occur in food and feed. They are potent hepatocarcinogens, and are known to cause immunosuppression. We investigated the effect of aflatoxin B(1) (AFB(1)), aflatoxin B(2) (AFB(2)) and aflatoxin G(1) (AFG(1)) exposure, alone and in combination, on the secretion of key pro- and anti-inflammatory cytokines from the murine macrophage cell line, J774A.1. Exposure of macrophages to low doses of aflatoxin (0.01 or 0.1ng/mL) resulted in a statistically significant change in the secretion of a number of cytokines following stimulation with lipopolysaccharide (LPS), a component of Gram-negative bacterial cell walls. Specifically, treatment with AFB(1) or AFB(2) alone significantly decreased (P<0.01) the secretion of the anti-inflammatory cytokine interleukin (IL) 10 (IL-10), while the secretion of the pro-inflammatory cytokine IL-6 was significantly increased (P<0.01). In addition, aflatoxin exposure affected expression levels of key cell surface markers involved in the inflammatory response. Toll-like receptor 2 (TLR2) and Cluster of Differentiation 14 (CD14) expression levels decreased significantly (P<0.01), but Toll-like receptor 4 (TLR4) expression was unaffected. This data provides further insight into the mechanisms by which aflatoxins modulate the host immune response to exert their immunosuppressive activity.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Christine E Loscher, Richard O'Kennedy, Edwina Stack, Johanna C Bruneau

Experts in our system

1
Christine E Loscher
Dublin City University
Total Publications: 30
 
2
R O'Kennedy
Dublin City University
Total Publications: 197