Type

Journal Article

Authors

Martin Clynes
Vincent Lynch
Padraig Doolan
Shirley O'Dea
Sinead Aherne
Helena Joyce
Joanne Keenan

Subjects

Biochemistry

Topics
anoikis olfm3 protein human ptk2 protein human gene expression profiling lung neoplasms real time polymerase chain reaction secretion metabolism extracellular matrix proteins humans glycoproteins gene expression cell cycle carcinoma squamous cell genetics focal adhesion kinase 1 casp3 protein human cell survival rna interference autophagy phosphorylation oligonucleotide array sequence analysis gene knockdown techniques caspase 3 cell line tumor

Olfactomedin III expression contributes to anoikis-resistance in clonal variants of a human lung squamous carcinoma cell line. (2011)

Abstract Three clonal subpopulations of DLKP, a poorly differentiated squamous lung carcinoma cell line, display striking differences in ability to survive in suspension (anoikis resistance). DLKP-SQ is anoikis resistant (7.5% anoikis at 24 h). In contrast, DLKP-M and DLKP-I are sensitive to anoikis (49.2% and 42.6% respectively). DLKP-I shows increased apoptosis consistently over all time points tested while DLKP-M appear to slow down metabolically and perhaps delays onset of anoikis by undergoing autophagy. Expression microarray analysis identified pronounced differential expression of Olfactomedin 3 (OLFM3) between the clones. High expression of OLFM3 was confirmed at the RNA level by qRT-PCR in DLKP-SQ and at the protein level by Western blotting (within the cell and secreted). Little or no OLFM3 was detected in the other two clones (DLKP-M and DLKP-I). Following siRNA knockdown of OLFM3 in DLKP-SQ, anoikis was increased 2.8-fold to 21% which was intermediate between the anoikis levels in DLKP-SQ and DLKP-M or DLKP-I. This knockdown correlated with increased apoptosis in suspension but not in attached culture conditions. Addition of recombinant OLFM3 reduced anoikis in DLKP-I. This is the first instance of OLFM3 being linked with anoikis resistance in a human cancer cell line.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Martin Clynes, Vincent Lynch, Padraig Doolan, Shirley O'Dea, Sinead Aherne, Helena Joyce, Joanne Keenan

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Vincent Lynch
Dublin City University
Total Publications: 10
 
3
Padraig Doolan
Dublin City University
Total Publications: 37
 
4
Shirley O'Dea
Maynooth University
Total Publications: 22
 
5
Sinéad T Aherne
Dublin City University
Total Publications: 17
 
6
Helena Joyce
Dublin City University
Total Publications: 10
 
7
Joanne Keenan
Dublin City University
Total Publications: 23