Type

Journal Article

Authors

Martin Clynes
Mark Leonard
Dana DiNino
Stephen F Madden
Paula Meleady
Niall Barron
Padraig Doolan
Colin Clarke

Subjects

Biochemistry

Topics
cricetinae genetics cricetulus computational biology gene expression regulation cho cells animals databases nucleic acid internet

CGCDB: a web-based resource for the investigation of gene coexpression in CHO cell culture. (2011)

Abstract Coexpression analysis is a powerful, widely used methodology for the investigation of underlying patterns in gene expression data. This "guilt-by-association" approach aims to find groups of genes with closely correlated expression profiles. Observation of consistent correlations across phenotypically diverse samples indicates that these genes have a shared function. We have recently described the application of weighted gene coexpression network analysis (WGCNA) to a 295 sample production CHO cell line microarray dataset and elucidated groups of genes related to growth rate and cell-specific productivity (Qp). In this study, we present the CHO gene coexpression database (CGCDB), a web-based system, designed specifically for researchers in the CHO community to provide user-friendly access to these gene-gene coexpression patterns. In addition to correlation between genes, the direct correlations between probesets and either growth rate or Qp are provided. Results are presented to the user via an interactive network diagram and in a downloadable tabular format. It is hoped that this resource will allow researchers to prioritize cell line engineering and/or biomarker candidates to enhance CHO-based cell culture for the production of biotherapeutics. Availability: www.cgcdb.org.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Martin Clynes, Mark Leonard, Dana DiNino, Stephen F Madden, Paula Meleady, Niall Barron, Padraig Doolan, Colin Clarke

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Stephen F Madden
Dublin City University
Total Publications: 42
 
3
Paula Meleady
Dublin City University
Total Publications: 95
 
4
Niall Barron
Dublin City University
Total Publications: 44
 
5
Padraig Doolan
Dublin City University
Total Publications: 37
 
6
Colin Clarke
Dublin City University
Total Publications: 26