Type

Journal Article

Authors

David E Williams
Stephen Daniels
Richard O'Kennedy
Bryony James
Ruairi Monaghan
Suresh Uppal
Vladimir Gubala
Colin Doyle
Cedric Volcke
Chandra K Dixit
and 2 others

Subjects

Chemistry

Topics
chemistry electroplating instrumentation surface plasmon resonance biological assay dna polymerization gold

Multi-layered plasma-polymerized chips for SPR-based detection. (2011)

Abstract The surface functionalization of a noble metal is crucial in a surface plasmon resonance-based biomolecular detection system because the interfacial coating must retain the activity of immobilized biomolecules while enhancing the optimal loading. We present here a one-step, room-temperature, high-speed, gas-phase plasma polymerization process for functionalizing gold substrates using siloxane as an adhesion layer and acrylic acid as a functional layer. Siloxane- and thiol-based coatings were compared for their performance as adhesion and the interfacial layer for subsequent functionalization. An in situ sequential deposition of siloxane and acrylic acid resulted in a 7-fold increase in carboxylic functionality surfacial content compared to films deposited with thiol-containing precursors. Grading of the layer composition achieved as a consequence of ion-induced mixing on the surface coating under the application of the plasma is confirmed through secondary ion mass spectroscopic studies. DNA hybridization assays were demonstrated on gold/glass substrates using surface plasmon enhanced ellipsometry and the applicability of this coating for protein immunoassays were demonstrated with plasma functionalized gold/plastic substrates in Biacore 3000 SPR instrument.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

David E Williams, Stephen Daniels, Richard O'Kennedy, Bryony James, Ruairi Monaghan, Suresh Uppal, Vladimir Gubala, Colin Doyle, Cedric Volcke, Chandra K Dixit and 2 others

Experts in our system

1
David E Williams
Dublin City University
Total Publications: 18
 
2
Stephen Daniels
Dublin City University
Total Publications: 64
 
3
R O'Kennedy
Dublin City University
Total Publications: 197
 
4
Ruairi Monaghan
Dublin City University
Total Publications: 4
 
5
Vladimir Gubala
Dublin City University
Total Publications: 17