Type

Journal Article

Authors

Martin Clynes
Mark Leonard
Mark W Melville
Patrick Gammell
Colin Clarke
Finbar O'Sullivan
Joanne Keenan
Niall Barron
Michael Henry
Padraig Doolan
and 1 others

Subjects

Microbiology

Topics
animals cho cells antibodies monoclonal biotechnology cell survival cricetulus proteome two dimensional difference gel electrophoresis standards chromatography liquid metabolism tandem mass spectrometry glucosephosphate dehydrogenase phenotype cricetinae blotting western recombinant proteins

Sustained productivity in recombinant Chinese hamster ovary (CHO) cell lines: proteome analysis of the molecular basis for a process-related phenotype. (2011)

Abstract The ability of mammalian cell lines to sustain cell specific productivity (Qp) over the full duration of bioprocess culture is a highly desirable phenotype, but the molecular basis for sustainable productivity has not been previously investigated in detail. In order to identify proteins that may be associated with a sustained productivity phenotype, we have conducted a proteomic profiling analysis of two matched pairs of monoclonal antibody-producing Chinese hamster ovary (CHO) cell lines that differ in their ability to sustain productivity over a 10 day fed-batch culture. Proteomic profiling of inherent differences between the two sets of comparators using 2D-DIGE (Difference Gel Electrophoresis) and LC-MS/MS resulted in the identification of 89 distinct differentially expressed proteins. Overlap comparisons between the two sets of cell line pairs identified 12 proteins (AKRIB8, ANXA1, ANXA4, EIF3I, G6PD, HSPA8, HSP90B1, HSPD1, NUDC, PGAM1, RUVBL1 and CNN3) that were differentially expressed in the same direction. These proteins may have an important role in sustaining high productivity of recombinant protein over the duration of a fed-batch bioprocess culture. It is possible that many of these proteins could be useful for future approaches to successfully manipulate or engineer CHO cells in order to sustain productivity of recombinant protein.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Martin Clynes, Mark Leonard, Mark W Melville, Patrick Gammell, Colin Clarke, Finbar O'Sullivan, Joanne Keenan, Niall Barron, Michael Henry, Padraig Doolan and 1 others

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Colin Clarke
Dublin City University
Total Publications: 26
 
3
Joanne Keenan
Dublin City University
Total Publications: 23
 
4
Niall Barron
Dublin City University
Total Publications: 44
 
5
Michael Henry
Dublin City University
Total Publications: 78
 
6
Padraig Doolan
Dublin City University
Total Publications: 37