Type

Journal Article

Authors

Brian D Maccraith
Richard O'Kennedy
Colm McAtamney
Michal Trnavsky
Robert Blue
Lourdes Basabe-Desmonts
Stephen Hearty
Barry McDonnell
Duncan Hill

Subjects

Biochemistry

Topics
equipment design fluorescence disposable equipment instrumentation avidin protein array analysis humans biotin c reactive protein

Novel disposable biochip platform employing supercritical angle fluorescence for enhanced fluorescence collection. (2011)

Abstract This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a planar substrate. The optical elements are based on supercritical angle fluorescence (SAF) which provides substantial enhancement of the fluorescence collection efficiency but also confines the fluorescence detection volume strictly to the immediate proximity of the biochip surface, thereby having the potential to discriminate against background fluorescence from the analyte solution. An optical reader is also described that enables interrogation and fluorescence collection from the nine optical elements on the chip. The sensitivity of the system was determined with a biotin-avidin assay while its clinical utility was demonstrated in an assay for C-reactive protein (CRP), an inflammation marker.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Brian D Maccraith, Richard O'Kennedy, Colm McAtamney, Michal Trnavsky, Robert Blue, Lourdes Basabe-Desmonts, Stephen Hearty, Barry McDonnell, Duncan Hill

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Lourdes Basabe-Desmonts
Dublin City University
Total Publications: 15
 
3
Stephen Hearty
Dublin City University
Total Publications: 27