Type

Journal Article

Authors

Martin Clynes
Joanne Keenan
Laura Breen

Subjects

Microbiology

Topics
dose response relationship drug cell line tumor clone cells pathology antineoplastic agents inhibitory concentration 50 lung neoplasms cell separation neoplasm invasiveness humans pharmacology drug resistance neoplasm drug effects

Generation of lung cancer cell line variants by drug selection or cloning. (2011)

Abstract Clonal variants or subpopulations have been isolated from every major histological type of cancer, and cellular heterogeneity in lung cancer is a common occurrence. These subpopulations may exhibit differences in drug resistance and invasive potential. One therefore needs to consider the subpopulations as well as the tumour to overcome the barriers of drug resistance and metastasis for successful treatment. Isogenic variants of cancer cell lines can be very valuable in providing controlled human experimental systems to study clinically relevant parameters such as drug resistance and invasiveness. These variants can be established by selection based on a characteristic of the subpopulation or by isolating clonal subpopulations from a heterogeneous population. Drug-resistant variants can be generated by pulse selection, which usually generates low-level resistance, which may as well be clinically relevant, or by continuous exposure, which can be used to obtain high-level resistant variants. Clonal subpopulations may also be isolated based on morphological differences using simple cell-culture-based techniques.
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Full list of authors on original publication

Martin Clynes, Joanne Keenan, Laura Breen

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Joanne Keenan
Dublin City University
Total Publications: 23
 
3
Laura Breen
Dublin City University
Total Publications: 11