Type

Journal Article

Authors

Richard O'Kennedy
Brian D Maccraith
Sandeep K Vashist
Chandra K Dixit

Subjects

Biochemistry

Topics
enzyme linked immunosorbent assay immunoglobulin g cattle surface plasmon resonance bicinchoninic acid ahsg protein mouse ahsg protein human polypropylenes quinolines serum albumin bovine mice alpha 2 hs glycoprotein humans blood proteins animals adsorption horseradish peroxidase immunology surface properties chemistry glass

Evaluation of apparent non-specific protein loss due to adsorption on sample tube surfaces and/or altered immunogenicity. (2011)

Abstract The non-specific loss of protein analytes can have a major effect on assay results particularly where the concentrations of such analytes are extremely low and the matrix is complex. This report assesses how the protein incubated in sample tubes may be lost due to adsorption. Use of proteins, such as bovine serum albumin (BSA), may be used to pre-treat tubes to reduce such losses. However, such losses may also be associated with structural perturbations leading to changes in immunogenicity (as a result of alterations in specific epitope-related conformations). This can lead to erroneous results or lack of comparability with a range of methodologies such as the bicinchoninic protein assay and immunoassays or when surface plasmon resonance (SPR)-based approaches are used. A model system to evaluate these phenomena is proposed.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Richard O'Kennedy, Brian D Maccraith, Sandeep K Vashist, Chandra K Dixit

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197