Type

Journal Article

Authors

Martin Clynes
Paul Dowling

Subjects

Biochemistry

Topics
cell line animals chemistry biological markers proteome culture media conditioned metabolism humans proteomics models biological secretion

Conditioned media from cell lines: a complementary model to clinical specimens for the discovery of disease-specific biomarkers. (2010)

Abstract In the strictest sense, the cell secretome (conditioned media) refers to the collection of proteins that contain a signal peptide and are processed via the endoplasmic reticulum and Golgi apparatus through the classical secretion pathway. More generally, the secretome also encompasses proteins shed from the cell surface and intracellular proteins released through non-classical secretion pathway or exosomes. These secreted proteins include numerous enzymes, growth factors, cytokines and hormones or other soluble mediators. They are fundamental in the processes of cell growth, differentiation, invasion and angiogenesis by regulating cell-to-cell and cell-to-extracellular matrix interactions. The main aim of this review is to provide a synopsis of findings from the analysis of the secretome taking diabetes, cancer and neurodegenerative diseases as examples. We will also discuss the preparation of conditioned media and on the main proteomic-based methodological approaches that have been developed for the study of secreted/shed proteins.
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Full list of authors on original publication

Martin Clynes, Paul Dowling

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Paul Dowling
Dublin City University
Total Publications: 79