Type

Journal Article

Authors

Martin Clynes
Mark Leonard
Mark Melville
Patrick Gammell
Finbarr O'Sullivan
Paula Meleady
Niall Barron
Padraig Doolan
Colin Clarke

Subjects

Biochemistry

Topics
cell line algorithms oligonucleotide array sequence analysis computational biology least squares analysis cricetinae gene expression phenotype animals gene expression profiling chromatography high pressure liquid cho cells cricetulus

Predicting cell-specific productivity from CHO gene expression. (2010)

Abstract Improving the rate of recombinant protein production in Chinese hamster ovary (CHO) cells is an important consideration in controlling the cost of biopharmaceuticals. We present the first predictive model of productivity in CHO bioprocess culture based on gene expression profiles. The dataset used to construct the model consisted of transcriptomic data from 70 stationary phase, temperature-shifted CHO production cell line samples, for which the cell-specific productivity had been determined. These samples were utilised to investigate gene expression over a range of high to low monoclonal antibody and fc-fusion-producing CHO cell lines. We utilised a supervised regression algorithm, partial least squares (PLS) incorporating jackknife gene selection, to produce a model of cell-specific productivity (Qp) capable of predicting Qp to within 4.44 pg/cell/day root mean squared error in cross model validation (RMSE(CMV)). The final model, consisting of 287 genes, was capable of accurately predicting Qp in a further panel of 10 additional samples which were incorporated as an independent validation. Several of the genes constituting the model are linked with biological processes relevant to protein metabolism.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Martin Clynes, Mark Leonard, Mark Melville, Patrick Gammell, Finbarr O'Sullivan, Paula Meleady, Niall Barron, Padraig Doolan, Colin Clarke

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Finbarr O'Sullivan
Dublin City University
Total Publications: 21
 
3
Paula Meleady
Dublin City University
Total Publications: 95
 
4
Niall Barron
Dublin City University
Total Publications: 44
 
5
Padraig Doolan
Dublin City University
Total Publications: 37
 
6
Colin Clarke
Dublin City University
Total Publications: 26