Type

Journal Article

Authors

R O'Kennedy
C J Hayes
P Leonard

Subjects

Microbiology

Topics
biosensing techniques immunology thermodynamics chemistry binding sites instrumentation antibodies monoclonal classification prostate specific antigen kinetics

Rapid temperature-dependent antibody ranking using Biacore A100. (2010)

Abstract The ability to select antibodies with stable thermodynamic profiles can greatly enhance the performance of point-of-care (POC) devices allowing reproducible "on-the-spot" analysis for markers of clinical and environmental relevance. We show that by ranking antibodies based on their kinetic profiles at two different temperatures, greater information content is acquired, facilitating the selection of antibodies with desired binding characteristics. Observed binding patterns highlight the importance of temperature to assay design, which should be fully taken into consideration to ensure that the appropriate antibody is selected for the desired test.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

R O'Kennedy, C J Hayes, P Leonard

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Paul Leonard
Dublin City University
Total Publications: 27