Type

Journal Article

Authors

Richard O'Kennedy
Martin Danaher
Jenny Fitzgerald
Paul Leonard
Elaine Darcy

Subjects

Biochemistry

Topics
antibodies monoclonal bacterial proteins chemistry isolation purification immunology antibodies animals staphylococcal protein a mice ammonium sulfate nerve tissue proteins g substrate ig l binding protein peptostreptococcus enzyme linked immunosorbent assay ligands chemical precipitation chromatography affinity humans

Purification of antibodies using affinity chromatography. (2010)

Abstract Affinity chromatography permits the isolation of a target analyte from a complex mixture and can be utilised to purify proteins, carbohydrates, drugs, haptens, or any analyte of interest once an affinity pair is available. It involves the exploitation of specific interactions between a binding affinity pair, such as those between an antibody and its associated antigen, or between any ligand and its associated binding receptor/protein. With the discovery of protein A in 1970, and, subsequently proteins G and L, immuno-affinity chromatography has grown in popularity and is now the standard methodology for the purification of antibodies which may be implemented for a selection of different applications such as immunodiagnostics. This chapter is designed to inform the researcher about the basic techniques involved in the affinity chromatography-based purification of monoclonal, polyclonal, and recombinant antibodies. Examples are provided for the use of proteins A and G. In addition, tables are provided that allow the reader to select the most appropriate protein for use in the isolation of their antibody.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Richard O'Kennedy, Martin Danaher, Jenny Fitzgerald, Paul Leonard, Elaine Darcy

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Martin Danaher
Teagasc
Total Publications: 58
 
3
Jenny Fitzgerald
Dublin City University
Total Publications: 18
 
4
Paul Leonard
Dublin City University
Total Publications: 27
 
5
Elaine Darcy
Dublin City University
Total Publications: 6