Type

Journal Article

Authors

Richard O'Kennedy
Elaine Darcy
Paul Leonard
Jenny Fitzgerald

Subjects

Microbiology

Topics
chemistry ligands antibodies immobilized immunology animals chromatography affinity humans antibody formation

Immunoaffinity chromatography. (2010)

Abstract Antibody-based separation methods, such as immunoaffinity chromatography (IAC), are powerful purification and isolation techniques. Antibodies isolated using these techniques have proven highly efficient in applications ranging from clinical diagnostics to environmental monitoring. IAC is an efficient antibody separation method which exploits the binding efficiency of a ligand to an antibody. Essential to the successful design of any IAC platform is the optimisation of critical experimental parameters such as: (a) the biological affinity pair, (b) the matrix support, (c) the immobilisation coupling chemistry, and (d) the effective elution conditions. These elements and the practicalities of their use are discussed in detail in this review. At the core of all IAC platforms is the high-affinity interactions between antibodies and their related ligands; hence, this review entails a brief introduction to the generation of antibodies for use in IAC and also provides specific examples of their potential applications.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Richard O'Kennedy, Elaine Darcy, Paul Leonard, Jenny Fitzgerald

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Elaine Darcy
Dublin City University
Total Publications: 6
 
3
Paul Leonard
Dublin City University
Total Publications: 27
 
4
Jenny Fitzgerald
Dublin City University
Total Publications: 18