Type

Journal Article

Authors

Richard O'Kennedy
Sushrut Arora
Caroline Viguier
Barry Byrne
Niamh Gilmartin
Paul Leonard
Katherine Welbeck

Subjects

Microbiology

Topics
animals female cattle chemistry milk immunology reference standards diagnosis surface plasmon resonance metabolism single chain antibodies enzyme linked immunosorbent assay biosensing techniques early diagnosis acetylglucosaminidase mastitis bovine humans biological markers automation laboratory

Generation of an anti-NAGase single chain antibody and its application in a biosensor-based assay for the detection of NAGase in milk. (2010)

Abstract Bovine mastitis, an inflammation of the mammary gland in cows, is a major challenge for the dairy industry worldwide as it lowers milk yield, reduces milk quality and increases overall production costs. Early diagnosis is of the utmost importance. N-acetyl-β-D-glucosaminidase (NAGase) is an enzyme released into milk during inflammation and acts as an early indicator of mastitis. This paper describes the selection of anti-NAGase single chain fragment variable antibodies (scFv) from naïve human antibody libraries and their incorporation into an automated optical biosensor-based immunoassay to detect NAGase in milk. The scFv with the highest affinity for NAGase was first characterized by inhibition ELISA, followed by further evaluation using a surface plasmon resonance platform. Purified NAGase was immobilized on the surface of a CM5 chip and spiked NAGase milk samples were analyzed. The limit of detection for the assay for the assay was determined as 1μg/ml.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Richard O'Kennedy, Sushrut Arora, Caroline Viguier, Barry Byrne, Niamh Gilmartin, Paul Leonard, Katherine Welbeck

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Sushrut Arora
Dublin City University
Total Publications: 6
 
3
Barry Byrne
Dublin City University
Total Publications: 15
 
4
Niamh Gilmartin
Dublin City University
Total Publications: 8
 
5
Paul Leonard
Dublin City University
Total Publications: 27