Type

Journal Article

Authors

Richard O'Kennedy
Brian D Maccraith
Brian O'Reilly
Feidhlim T O'Neill
Sandeep Kumar Vashist
Chandra Kumar Dixit

Subjects

Microbiology

Topics
polymers biological markers humans antibodies immobilized ahsg protein human chemistry alpha 2 hs glycoprotein atherosclerosis silanes immunology carcinoma hepatocellular enzyme linked immunosorbent assay diagnosis liver neoplasms blood proteins

Development of a high sensitivity rapid sandwich ELISA procedure and its comparison with the conventional approach. (2010)

Abstract A highly sensitive and rapid sandwich enzyme-linked immunosorbent assay (ELISA) procedure was developed for the detection of human fetuin A/AHSG (alpha2-HS-glycoprotein), a specific biomarker for hepatocellular carcinoma and atherosclerosis. Anti-human fetuin A antibody was immobilized on aminopropyltriethoxysilane-mediated amine-functionalized microtiter plates using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride and N-hydroxysulfosuccinimide-based heterobifunctional cross-linking. The analytical sensitivity of the developed assay was 39 pg/mL, compared to 625 pg/mL for the conventional assay. The generic nature of the developed procedure was demonstrated by performing human fetuin A assays on different polymeric matrixes, i.e., polystyrene, poly(methyl methacrylate), and polycyclo-olefin (Zeonex), in a modified microtiter plate format. Thus, the newly developed procedure has considerable advantages over the existing method.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Richard O'Kennedy, Brian D Maccraith, Brian O'Reilly, Feidhlim T O'Neill, Sandeep Kumar Vashist, Chandra Kumar Dixit

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197