Type

Journal Article

Authors

Antonio J. Ricco
Richard O'Kennedy
Louise M Barrett
Jose L. Garcia-Cordero

Subjects

Medicine & Nursing

Topics
pathology cytology microfluidic analytical techniques point of care systems mastitis bovine standards milk quality control cytological techniques economics instrumentation time factors cell count adipocytes cattle animals metabolism diagnosis

Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring. (2010)

Abstract We report a rapid, low-cost, portable microfluidic sedimentation cytometer (SeCy) for assessing the somatic cell count and fat content of milk in 15 min using a "sample-in, answer-out" approach. The system consists of 12 independent microfluidic devices, essentially flattened funnel structures, fabricated on the footprint of a single plastic compact disc (CD). Each funnel structure holds 150 μL of milk, has an inlet for milk filling and an outlet for air to escape, and ends in a narrow, closed-end microfluidic channel that facilitates packing of the cells into a column whose length is proportional to cell count. The closed-end channel provides accurate cell counts over the range 50,000->3,000,000 cells per mL. The assay separates cells and fat globules based on their densities (by differential sedimentation), concentrating white cells in the closed-end channel near the outer rim of the CD for estimation of total "cell pellet" volume, while fat globules move toward the center of disc rotation, forming a fat "band" in the funnel. After adding milk to two or more microfluidic devices, the CD is loaded onto a custom-built reader unit that spins the disc for 15 min. Two low-cost microscopes in the reader image the centrifuged cell pellet and the fat band, providing a sufficiently accurate cell count to diagnose mastitis and measuring fat content as an indication of health and nutritional status.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Antonio J. Ricco, Richard O'Kennedy, Louise M Barrett, Jose L. Garcia-Cordero

Experts in our system

1
Antonio J. Ricco
Dublin City University
Total Publications: 23
 
2
R O'Kennedy
Dublin City University
Total Publications: 197