Type

Journal Article

Authors

Gillian P McMahon
Fiona Regan
Robert O'Connor
Shane Bermingham

Subjects

Biochemistry

Topics
chemistry pharmaceutical chromatography high pressure liquid chromatography daunorubicin sensitivity and specificity anthracyclines epirubicin blood doxorubicin antineoplastic agents calibration humans docetaxel paclitaxel taxoids blood proteins chemistry

Simultaneous determination of anthracyclines and taxanes in human serum using online sample extraction coupled to high performance liquid chromatography with UV detection. (2010)

Abstract An online SPE-LC method that can determine both anthracyclines and taxanes simultaneously in human serum samples is reported. The entire method of extraction, separation and UV detection was achieved online by column switching between an SPE column (Biotrap 500 (20 x 4 mm)) and an analytical column (Zorbax XDB C18, 150 x 4.6 mm, 5 microm) with a 23 min total cycle time. The method is linear (r(2)>0.998) over the range of 0.5-25 microg/mL. The analytes of interest are retained on the SPE column with good recovery (84-117%), while proteins and other serum components elute to waste. This online clean-up is much faster (150 s) and less manual than traditional off-line extraction methods. Using 0.1 mL spiked serum samples, the LOQ was 0.5 microg/mL. Intra- and inter-day precision were acceptable (< or = 15% RSD) at and above the LOQ. The method was applied to the analysis of serum samples from patients undergoing chemotherapy with these agents.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Gillian P McMahon, Fiona Regan, Robert O'Connor, Shane Bermingham

Experts in our system

1
Fiona Regan
Dublin City University
Total Publications: 115
 
2
Robert O'Connor
Dublin City University
Total Publications: 74