Type

Journal Article

Authors

Martin Clynes
Timothy Charlebois
Mark Leonard
Kevin McCarthy
Martin Sinacore
Mark Melville
Patrick Gammell
Ross Gallagher
Michael Henry
Niall Barron
and 2 others

Subjects

Biochemistry

Topics
cricetinae epithelial cells up regulation gene expression profiling cdc48 protein cell cycle proteins adenosine triphosphatases biosynthesis rna small interfering physiology cricetulus metabolism proteome gene expression cho cells animals gene silencing cell cycle

Microarray and proteomics expression profiling identifies several candidates, including the valosin-containing protein (VCP), involved in regulating high cellular growth rate in production CHO cell lines. (2010)

Abstract A high rate of cell growth (micro) leading to rapid accumulation of viable biomass is a desirable phenotype during scale up operations and the early stages of production cultures. In order to identify genes and proteins that contribute to higher growth rates in Chinese hamster ovary (CHO) cells, a combined approach using microarray and proteomic expression profiling analysis was carried out on two matched pairs of CHO production cell lines that displayed either fast or slow growth rates. Statistical analysis of the microarray and proteomic data separately resulted in the identification of 118 gene transcripts and 58 proteins that were differentially expressed between the fast- and slow-growing cells. Overlap comparison of both datasets identified a priority list of 21 candidates associated with a high growth rate phenotype in CHO. Functional analysis (by siRNA) of five of these candidates identified the valosin-containing protein (VCP) as having a substantial impact on CHO cell growth and viability. Knockdown of HSPB1 and ENO1 also had an effect on cell growth (negative and positive, respectively). Further functional validation in CHO using both gene knockdown (siRNA) and overexpression (cDNA) confirmed that altered VCP expression impacted CHO cell proliferation, indicating that VCP and other genes and proteins identified here may play an important role in the regulation of CHO cell growth during log phase culture and are potential candidates for CHO cell line engineering strategies.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Martin Clynes, Timothy Charlebois, Mark Leonard, Kevin McCarthy, Martin Sinacore, Mark Melville, Patrick Gammell, Ross Gallagher, Michael Henry, Niall Barron and 2 others

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Michael Henry
Dublin City University
Total Publications: 78
 
3
Niall Barron
Dublin City University
Total Publications: 44