Type

Journal Article

Authors

N Barron
M Clynes
P Gammell
P Doolan
P Meleady
M Henry
P Dowling
M K Muniyappa

Subjects

Biochemistry

Topics
neoplasm proteins reverse transcriptase polymerase chain reaction humans mirn29 microrna human ran protein human cancer invasion protein expression rna small interfering lung cancer cells down regulation micrornas genetics proteomics metabolism gene expression regulation neoplastic cancer cell lines phenotype pancreatic cancer pathology gene knockdown techniques biosynthesis carcinoma non small cell lung cell line tumor cell invasion ran gtp binding protein rna messenger cell lines lung neoplasms neoplasm invasiveness gene expression profiling transfection cell proliferation

MiRNA-29a regulates the expression of numerous proteins and reduces the invasiveness and proliferation of human carcinoma cell lines. (2009)

Abstract In this study we have identified a functional role for miR-29a in cancer cell invasion and proliferation. MiRNA expression profiling of human NSCLC cell lines indicated that miR-29a levels were reduced in more invasive cell lines. Exogenous overexpression of miR-29a in both lung and pancreatic cancer cell lines resulted in a significant reduction in the invasion phenotype, as well as in proliferation. 2D DIGE proteomic profiling of cells transfected with pre-miR-29a or anti-miR-29a resulted in the identification of over 100 differentially regulated proteins. The fold change of protein expression was generally modest--in the range 1.2-1.7-fold. Only 14 were predicted computationally to have miR-29a seed sequences in their 3' UTR region. Subsequent studies using siRNA to knock down several candidate proteins from the 2D DIGE experiment identified RAN (a member of the RAS oncogene family) which significantly reduced the invasive capability of a model lung cancer cell line. We conclude that miR-29a has a significant anti-invasive and anti-proliferative effect on lung cancer cells in vitro and functions as an anti-oncomir. This function is likely mediated through the post-transcriptional fine tuning of the cellular levels of several proteins, both directly and indirectly, and in particular we provide some evidence that RAN represents one of these.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Dublin City University -> PubMed
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

N Barron, M Clynes, P Gammell, P Doolan, P Meleady, M Henry, P Dowling, M K Muniyappa

Experts in our system

1
Niall Barron
Dublin City University
Total Publications: 44
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Padraig Doolan
Dublin City University
Total Publications: 37
 
4
Paula Meleady
Dublin City University
Total Publications: 95
 
5
Michael Henry
Dublin City University
Total Publications: 78
 
6
Paul Dowling
Dublin City University
Total Publications: 79