Type

Journal Article

Authors

Richard O'Kennedy
Bernard Bradshaw
Sharon Stapleton

Subjects

Microbiology

Topics
enzyme linked immunosorbent assay surface plasmon resonance corynebacterium pseudotuberculosis diagnosis goats veterinary blood sheep diseases phospholipase d antibodies bacterial corynebacterium infections metabolism biosensing techniques animals recombinant proteins immunology sheep reagent kits diagnostic

Development of a surface plasmon resonance-based assay for the detection of Corynebacterium pseudotuberculosis infection in sheep. (2009)

Abstract Caseous lymphadenitis (CLA), a disease affecting sheep and goats, is caused by Corynebacterium pseudotuberculosis and is difficult to detect, especially at early stages in its development. A surface plasmon resonance-based biosensor assay for the detection of antibodies to the phospholipase D (PLD) exotoxin of C. pseudotuberculosis in sheep serum was successfully generated. It employed a recombinant form of PLD, which was immobilised, and all aspects of the assay including minimisation of non-specific binding, and the regeneration of the chip, were optimised. The applicability of the assay was initially demonstrated using sera collected from experimentally infected sheep and from sheep with no prior history of infection. The assay was then evaluated on a panel of clinical samples and the results obtained compared very favourably to those obtained by a double sandwich ELISA (over 90% similarity) and clearly verified its analytical value.
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Full list of authors on original publication

Richard O'Kennedy, Bernard Bradshaw, Sharon Stapleton

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197