Journal Article


Martin Clynes
Patrick Gammell
Niraj Kumar



production methods cell lines therapeutic use cell culture product lines cell proliferation cell cycle arrest chinese hamster ovary cells

Proliferation control strategies to improve productivity and survival during CHO based production culture : A summary of recent methods employed and the effects of proliferation control in product secreting CHO cell lines. (2006)

Abstract Chinese Hamster Ovary cells are the primary system for the production of recombinant proteins for therapeutic use. Protein productivity is directly proportional to viable biomass, viability and culture longevity of the producer cells and a number of approaches have been taken to optimise these parameters. Cell cycle arrest, particularly in G1 phase, typically using reduced temperature cultivation and nutritional control have been used to enhance productivity in production cultures by prolonging the production phase, but the mechanism by which these approaches work is still not fully understood. In this article, we analyse the public literature on proliferation control approaches as they apply to production cell lines with particular reference to what is known about the mechanisms behind each approach.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Martin Clynes, Patrick Gammell, Niraj Kumar

Experts in our system

Martin Clynes
Dublin City University
Total Publications: 209