Type

Journal Article

Authors

Martin Clynes
Lorraine O'Driscoll
Per Jeppesen
Michael Henry
Paula Meleady
Sweta Rani
William Shields
Paul Dowling

Subjects

Biochemistry

Topics
panel reveals insulin beta cell phenotypes metabolism chromogranins blotting western secretogranin iii phenotype culture media conditioned mass spectrometry insulin secreting cells gel electrophoresis media pharmacology proteome electrophoresis gel two dimensional animals glucose cell function prealbumin cells cultured mice cytology drug effects insulin secretion proteins analysis cell culture secretion

Proteomic analysis of conditioned media from glucose responsive and glucose non-responsive phenotypes reveals a panel of secreted proteins associated with beta cell dysfunction. (2008)

Abstract Media conditioned by dysfunctioning pancreatic beta cells offer an excellent source of potential protein markers associated with this phenotype. Proteins identified from cell culture model systems are often found to be of importance clinically. Previous work by us and others have shown that low-passage MIN-6 cells (MIN-6(L)) respond to changes in glucose concentrations, producing an approximately 5.5-fold glucose-stimulated insulin secretion (GSIS) in response to 26.7 mmol/L, compared with 3.3 mmol/L, glucose. After continuous culture or high-passage (MIN-(H)), this GSIS was no longer present and thus represents an excellent model system for investigating beta cell dysfunction. Employing 2-D difference gel electrophoresis and mass spectrometry a panel of protein markers were identified in conditioned media (CM) from MIN-6(L) and MIN-6(H) beta cells. These proteins, including secretogranin II, secretogranin III and transthyretin, are associated with secretory granule biogenesis and were found to have substantially increased levels in the CM from the non-responsive high-passage MIN-6 beta cells. A panel of protein markers found to have increased abundance levels in CM from MIN-6(H) compared with MIN-6(L) beta cells may have the potential to be used clinically for assessing beta cell function and to monitor the effects of specific therapeutics.
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Full list of authors on original publication

Martin Clynes, Lorraine O'Driscoll, Per Jeppesen, Michael Henry, Paula Meleady, Sweta Rani, William Shields, Paul Dowling

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 152
 
3
Michael Henry
Dublin City University
Total Publications: 78
 
4
Paula Meleady
Dublin City University
Total Publications: 95
 
5
Sweta Rani
Trinity College Dublin
Total Publications: 22
 
6
Paul Dowling
Dublin City University
Total Publications: 79