Journal Article


Martin Clynes
Aongus Curran
Michael Henry
Paula Meleady
Robert Wormald
Paul Dowling



proteomics pathology early detection saliva proteins analysis heavy chain patients carcinoma squamous cell mass spectrometry 2d dige cofilin 1 humans metabolism lymphatic metastasis calcium binding protein head and neck neoplasms proteome female gene expression regulation neoplastic tumor markers biological neoplasm proteins

Analysis of the saliva proteome from patients with head and neck squamous cell carcinoma reveals differences in abundance levels of proteins associated with tumour progression and metastasis. (2008)

Abstract The objective of this study was to identify differentially expressed proteins in saliva from HNSCC patients compared to a control group. Saliva samples from eight individuals with non-malignant conditions of the head and neck region were employed as a control group and compared to saliva from eight patients with HNSCC using 2D DIGE analysis and subsequent mass spectrometry identification of candidate proteins. Beta fibrin (+2.77-fold), S100 calcium binding protein (+5.35-fold), transferrin (+3.37-fold), immunoglobulin heavy chain constant region gamma (+3.28) and cofilin-1 (+6.42) were all found to be significantly increased in the saliva from HNSCC samples compared to the control group whereas transthyretin (-2.92-fold) was significantly decreased. The increased abundance of one of the proteins identified (S100 calcium binding protein) was confirmed by immunoblot analysis. Many of these proteins are involved in tumour progression, metastasis and angiogenesis. The proximity of saliva to the developing tumour is undoubtedly a major factor in facilitating detection of these proteins and such a strategy may lead to the development of a panel of biomarkers useful for therapeutic monitoring and for early detection of HNSCC.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Dublin City University -> PubMed
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

Martin Clynes, Aongus Curran, Michael Henry, Paula Meleady, Robert Wormald, Paul Dowling

Experts in our system

Martin Clynes
Dublin City University
Total Publications: 209
Michael Henry
Dublin City University
Total Publications: 78
Paula Meleady
Dublin City University
Total Publications: 95
Paul Dowling
Dublin City University
Total Publications: 79