Type

Journal Article

Authors

Martin Clynes
Timothy Charlebois
Mark Leonard
Linda Francullo
Mark Melville
Martin Sinacore
Padraig Doolan
Patrick Gammell
Michael Henry
Paula Meleady

Subjects

Biochemistry

Topics
animals gene expression profiling spectrometry proteome transfection cricetulus mass cell line matrix assisted laser bone morphogenetic proteins cytology cricetinae humans recombinant proteins gene expression recombinant human bone morphogenetic protein 2 bone morphogenetic protein 2 transforming growth factor beta chemistry genetics clone cells metabolism cho cells proteomics peptide mapping isolation purification cell culture techniques electrophoresis gel two dimensional physiology furin protein human dimerization furin

Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity following co-expression of PACEsol. (2008)

Abstract Chinese hamster ovary (CHO) cells are widely used for the production of recombinant protein biopharmaceuticals. The purpose of this study was to investigate differences in the proteome of CHO DUKX cells expressing recombinant human bone morphogenetic protein-2 (rhBMP-2) (G5 cells) compared to cells also expressing soluble exogenous paired basic amino acid cleaving enzyme soluble paired basic amino acid cleaving enzyme (PACEsol) (3C9 cells), which has been previously found to improve the post-translational processing of the mature rhBMP-2 dimer. PACEsol co-expression was also associated with a significant increase (almost four-fold) in cellular productivity of rhBMP-2 protein. Differential proteomic expression profiling using 2-D DIGE and MALDI-TOF MS was performed to compare 3C9 and G5 cells, and revealed a list of 60 proteins that showed differential expression (up/downregulated), with a variety of different cellular functions. A substantial number of these altered proteins were found to have chaperone activity, involved with protein folding, assembly and secretion, as well as a number of proteins involved in protein translation. These results support the use of proteomic profiling as a valuable tool towards understanding the biology of bioprocess cultures.
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Full list of authors on original publication

Martin Clynes, Timothy Charlebois, Mark Leonard, Linda Francullo, Mark Melville, Martin Sinacore, Padraig Doolan, Patrick Gammell, Michael Henry, Paula Meleady

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Padraig Doolan
Dublin City University
Total Publications: 37
 
3
Michael Henry
Dublin City University
Total Publications: 78
 
4
Paula Meleady
Dublin City University
Total Publications: 95