Type

Journal Article

Authors

Martin Clynes
Donal O'Gorman
Brendan O'Daly
Arnold Hill
Patrick Gammell
Helena Joyce
Padraig Doolan
Jai Prakash Mehta
Elaine Kenny
Lorraine O'Driscoll

Subjects

Microbiology

Topics
middle aged relevance aged rna neoplasm breast cancer patients gene expression regulation neoplastic oligonucleotide array sequence analysis global breast neoplasms genome aged 80 and over gene expression profiling genome human standards rna messenger isolation purification adult metabolism genes neoplasm humans pcr feasibility studies reverse transcriptase polymerase chain reaction female cluster analysis quantitative serum genetics reproducibility of results dna neoplasm blood

Feasibility and relevance of global expression profiling of gene transcripts in serum from breast cancer patients using whole genome microarrays and quantitative RT-PCR. (2008)

Abstract Previous studies, by ourselves and others, have indicated that gene transcripts are detectable extracellularly. Advancing on this work, in order to investigate the feasibility of analysing global gene expression profiles and so the possibility in the future of identifying panels of circulating mRNA biomarkers that may be diagnostic, prognostic or predictive for cancer, here we performed the first whole genome microarray analysis of human serum. RNA was isolated from pre-surgery serum and corresponding breast tumour and normal tissue biopsies, and from post-surgery and normal control serum. Specimens were examined using Affymetrix whole genome microarrays and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). Of the 54,675 mRNAs/variants analysed, approximately 8% and 45% were called Present in serum and breast tissue specimens, respectively. Differentially expressed genes were identified for each group of specimens analysed. Analysis, by qRT-PCR, of 3 selected transcripts further indicated that the nucleic acids detected were mRNA, not DNA. mRNAs are apparently present in serum and their global detection and identification can be successfully achieved using microarray technologies. The potential implication of this novel finding is that using microarrays it may be possible to identify a panel of extracellular mRNAs that are diagnostic, prognostic and/or predictive of outcome for cancer patients.
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Full list of authors on original publication

Martin Clynes, Donal O'Gorman, Brendan O'Daly, Arnold Hill, Patrick Gammell, Helena Joyce, Padraig Doolan, Jai Prakash Mehta, Elaine Kenny, Lorraine O'Driscoll

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Donal J O'Gorman
Dublin City University
Total Publications: 42
 
3
Arnold D K Hill
Royal College of Surgeons in Ireland
Total Publications: 109
 
4
Helena Joyce
Dublin City University
Total Publications: 10
 
5
Padraig Doolan
Dublin City University
Total Publications: 37
 
6
Jai Prakash Mehta
University College Dublin
Total Publications: 29
 
7
Elaine Kenny
Trinity College Dublin
Total Publications: 28
 
8
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 152