Type

Journal Article

Authors

Paula Meleady
Martin Clynes
Paul Dowling
Patrick Gammell
Priyanka Maurya
Niraj Kumar

Subjects

Microbiology

Topics
proteins spectrometry animals proteomics mass cho cells cricetulus protein array analysis reproducibility of results matrix assisted laser cricetinae

Proteomic profiling of secreted proteins from CHO cells using Surface-Enhanced Laser desorption ionization time-of-flight mass spectrometry. (2007)

Abstract Chinese hamster ovary (CHO) cells are the most commonly used host cell line for the production of recombinant biopharmaceuticals. These biopharmaceuticals are typically secreted from CHO cells and purified from harvested cell culture media. The purpose of this study was to investigate changes in the secreted proteome of CHO cells over the various stages of the growth cycle using Surface Enhanced Laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS). Conditioned media samples were collected each day over a 6 day growth period from CHO-K1 cells grown in low serum (0.5% FBS) conditions in monolayer culture. Samples were profiled on a number of ProteinChip arrays with different chromatographic surfaces. From this study, 24 proteins were found to be differentially regulated at different phases of the growth cycle in CHO-K1 cells, when profiled on two chromatographic surfaces, Q10 (anionic) and IMAC30 (metal affinity) ProteinChip arrays.
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Full list of authors on original publication

Paula Meleady, Martin Clynes, Paul Dowling, Patrick Gammell, Priyanka Maurya, Niraj Kumar

Experts in our system

1
Paula Meleady
Dublin City University
Total Publications: 95
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Paul Dowling
Dublin City University
Total Publications: 79