Type

Journal Article

Authors

Martin Clynes
John Crown
Michael Moriarty
Jo Ballot
Shunil Roy
Michael Henry
Paula Meleady
Lorraine O'Driscoll
Paul Dowling

Subjects

Microbiology

Topics
lung cancer chemical fractionation 2 d dige adult humans proteins image processing computer assisted carcinoma squamous cell chemistry down regulation early detection metabolism chromatography liquid proteomics up regulation blood male tumor markers biological electrophoresis gel two dimensional mass spectrometry middle aged aged haptoglobins alpha 2 hs glycoprotein reference standards lung carcinoma lung neoplasms isolation purification gel electrophoresis isoelectric focusing blood proteins

2-D difference gel electrophoresis of the lung squamous cell carcinoma versus normal sera demonstrates consistent alterations in the levels of ten specific proteins. (2007)

Abstract Most lung cancers are diagnosed too late for curative treatment to be possible, therefore early detection is crucial. Serum proteins are a rich source of biomarkers and have the potential to be used as diagnostic and prognostic indicators for lung cancer. In order to examine differences in serum levels of specific proteins associated with human lung squamous carcinoma, immunodepletion of albumin and five other high-abundant serum proteins followed by 2-D difference gel electrophoresis (DIGE) analysis and subsequent MS was used to generate a panel of proteins found to be differentially expressed between the cancer and normal samples. Proteins found to have increased abundance levels in squamous cell carcinoma sera compared to normal sera included apolipoprotein A-IV precursor, chain F; human complement component C3c, haptoglobin, serum amyloid A protein precursor and Ras-related protein Rab-7b. Proteins found to have lower abundance levels in squamous cell carcinoma sera compared to normal sera included alpha-2-HS glycoprotein, hemopexin precursor, proapolipoprotein, antithrombin III and SP40; 40. The data presented here demonstrate that high-abundant protein removal combined with 2-D DIGE is a powerful strategy for the discovery of potential biomarkers. The identification of lung cancer-specific biomarkers is crucial to early detection, which in turn could lead to a dramatic increase in survival rates.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Dublin City University -> PubMed
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

Martin Clynes, John Crown, Michael Moriarty, Jo Ballot, Shunil Roy, Michael Henry, Paula Meleady, Lorraine O'Driscoll, Paul Dowling

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
John Crown
Dublin City University
 
3
Michael Moriarty
Dublin City University
Total Publications: 8
 
4
Michael Henry
Dublin City University
Total Publications: 78
 
5
Paula Meleady
Dublin City University
Total Publications: 95
 
6
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 152
 
7
Paul Dowling
Dublin City University
Total Publications: 79