Type

Journal Article

Authors

Martin Clynes
Finbarr O'Sullivan
Niall Barron
Michael Kasper
Lorraine O'Driscoll
Eadaoin McKiernan

Subjects

Biochemistry

Topics
neuroglia extracellular matrix proteins embryonic stem cells islets of langerhans animals metabolism neurons mice cytology cell culture techniques phenotype tissue engineering cell line cell differentiation

Directed differentiation of mouse embryonic stem cells into pancreatic-like or neuronal- and glial-like phenotypes. (2007)

Abstract The derivation of definitive endoderm and in particular endocrine cell types from undifferentiated embryonic stem (ES) cells remains difficult to achieve. In this study, we investigated the potential to regulate the differentiation of ES cells into endodermal derivatives using extracellular factors previously associated with various aspects of pancreatic development. Feeder-free-cultured mouse ESD3 cells were manipulated to form embryoid bodies (EBs) in the presence of retinoic acid (RA). RA-treated EBs were subsequently exposed to sodium butyrate (SB), betacellulin (BTC) or activin A (AA). A comparative analysis was performed on these models of directed differentiation in parallel with a model of spontaneous differentiation. Lineage differentiation was determined by profiling multilineage marker transcript expression (neuronal, myogenic, exocrine and endocrine pancreas, extraembryonic and apoptotic) and subsequent protein expression within ES-derived cultures. Using a two-stage differentiation protocol developed during this study, we successfully demonstrated the derivation of an intermediate multipotential population (RA_EBs) from undifferentiated ES cells that preferentially gives rise to pancreatic endocrine insulin-expressing cell types in the presence of SB, and neuronal- and glial-like cell types in the presence of AA or BTC.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Martin Clynes, Finbarr O'Sullivan, Niall Barron, Michael Kasper, Lorraine O'Driscoll, Eadaoin McKiernan

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Finbarr O'Sullivan
Dublin City University
Total Publications: 21
 
3
Niall Barron
Dublin City University
Total Publications: 44
 
4
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 152