Journal Article


M Clynes
M Henry
A J Dowd
S A Glynn
P Meleady
P Maurya
P Dowling



neoplasm invasiveness spectrometry seldi tof ms peptide fragments drug resistance neoplasm tumor markers biological pharmacology tandem mass spectrometry spectrometry mass matrix assisted laser desorption ionization matrix assisted laser breast neoplasms cell lines transferrin mass paclitaxel serum free media isolation purification humans adult metabolism time of flight mass spectrometry neoplasm proteins female chemistry drug therapy cell line tumor culture media conditioned receptors transferrin cancer cells biosynthesis pathology maldi tof

Purification and identification of a 7.6-kDa protein in media conditioned by superinvasive cancer cells. (2007)

Abstract Selection of the human drug sensitive and invasive cell line (MDA-MB-435S-F) with the chemotherapeutic agent paclitaxel, resulted in the development of drug resistant cell lines displaying enhanced invasion-related characteristics. Serum-free conditioned media from the human cancer drug-sensitive and invasive cell line (MDA-MB-435S-F) and its paclitaxel-resistant superinvasive variant (MDA-MB-435S-F/Taxol10p4pSI) were analyzed using Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). A differentially expressed protein was observed at 7.6 kDa, which was 4-fold up-regulated in MDA-MB-435S-F/Taxol10p4pSI. The differentially expressed protein was identified using matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS), as a fragment of bovine transferrin. The transferrin receptor was also found to be overexpressed in the superinvasive cell line. Cleavage of serum proteins such as transferrin could provide a valuable source of markers for malignant tumours and could also play a role in aspects of cancer pathogenesis, such as tumour cachexia.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Dublin City University -> PubMed
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

M Clynes, M Henry, A J Dowd, S A Glynn, P Meleady, P Maurya, P Dowling

Experts in our system

Martin Clynes
Dublin City University
Total Publications: 209
Michael Henry
Dublin City University
Total Publications: 78
Sharon A Glynn
National University of Ireland Galway
Total Publications: 21
Paula Meleady
Dublin City University
Total Publications: 95
Paul Dowling
Dublin City University
Total Publications: 79