Type

Journal Article

Authors

Padraig Doolan
Martin Clynes
Niall Barron
Lorraine O'Driscoll
Jai Prakash Mehta

Subjects

Biochemistry

Topics
cell models clinical medicine metabolism gene expression regulation neoplastic humans oligonucleotide array sequence analysis genes cdc cell line tumor breast neoplasms multigene family microarray genetics receptors estrogen breast cancer cancer cell pathology biosynthesis gene expression profiling cell line

A microarray approach to translational medicine in breast cancer: how representative are cell line models of clinical conditions? (2007)

Abstract The aim of this study was to examine whether the degree to which cell lines model corresponding cells in vivo is an important aspect of their value as models in studying disease processes. The work presented here utilizes gene expression data from two published microarray datasets to compare the differences and similarities among the two systems in order to identify major transcriptional changes in the adaptation process from a tissue to a cell line. Gene ontology and pathway analyses of comparator gene lists showed that the cell cycle related genes were significantly up-regulated in cell lines and immune response related genes were significantly up-regulated in clinical specimens. Estrogen receptor analysis also indicated differences in the clustering patterns of cell lines relative to clinical specimens. These findings suggest that significant differences in gene expression exist between clinical conditions and their respective cell line models and that these differences should be taken into account when extrapolating cell line results to in vivo systems.
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Full list of authors on original publication

Padraig Doolan, Martin Clynes, Niall Barron, Lorraine O'Driscoll, Jai Prakash Mehta

Experts in our system

1
Padraig Doolan
Dublin City University
Total Publications: 37
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Niall Barron
Dublin City University
Total Publications: 44
 
4
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 152
 
5
Jai Prakash Mehta
University College Dublin
Total Publications: 29