Type

Journal Article

Authors

Martin Clynes
Nicholas Walsh
Norma O'Donovan
Helena Joyce
Patrick Gammell
Eoin Ryan
Jai Prakash Mehta
Eadaoin McKiernan
Padraig Doolan
Jason McMorrow
and 1 others

Subjects

Microbiology

Topics
male carcinoma basal cell microarrays standards skin polymerase chain reaction oligonucleotide array sequence analysis middle aged profile reproducibility of results humans aged aged 80 and over molecular cluster analysis female case control studies statistics as topic gene expression profiling genome metabolism

Investigation of the molecular profile of basal cell carcinoma using whole genome microarrays. (2006)

Abstract Skin cancer accounts for 1/3 of all newly diagnosed cancer. Although seldom fatal, basal cell carcinoma (BCC) is associated with severe disfigurement and morbidity. BCC has a unique interest for researchers, as although it is often locally invasive, it rarely metastasises. This paper, reporting the first whole genome expression microarray analysis of skin cancer, aimed to investigate the molecular profile of BCC in comparison to non-cancerous skin biopsies. RNA from BCC and normal skin specimens was analysed using Affymetrix whole genome microarrays. A Welch t-test was applied to data normalised using dCHIP to identify significant differentially-expressed genes between BCC and normal specimens. Principal component analysis and support vector machine analysis were performed on resulting genelists, Genmapp was used to identify pathways affected, and GOstat aided identification of areas of gene ontology more highly represented on these lists than would be expected by chance. Following normalisation, specimens clustered into groups of BCC specimens and of normal skin specimens. Of the 54,675 gene transcripts/variants analysed, 3,921 were differentially expressed between BCC and normal skin specimens. Of these, 2,108 were significantly up-regulated and 1,813 were statistically significantly down-regulated in BCCs. Functional gene sets differentially expressed include those involved in transcription, proliferation, cell motility, apoptosis and metabolism. As expected, members of the Wnt and hedgehog pathways were found to be significantly different between BCC and normal specimens, as were many previously undescribed changes in gene expression between normal and BCC specimens, including basonuclin2 and mrp9. Quantitative-PCR analysis confirmed our microarray results, identifying novel potential biomarkers for BCC.
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Full list of authors on original publication

Martin Clynes, Nicholas Walsh, Norma O'Donovan, Helena Joyce, Patrick Gammell, Eoin Ryan, Jai Prakash Mehta, Eadaoin McKiernan, Padraig Doolan, Jason McMorrow and 1 others

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Norma O'Donovan
Dublin City University
Total Publications: 52
 
3
Helena Joyce
Dublin City University
Total Publications: 10
 
4
Eoin Ryan
University College Dublin
 
5
Jai Prakash Mehta
University College Dublin
Total Publications: 29
 
6
Padraig Doolan
Dublin City University
Total Publications: 37