Type

Journal Article

Authors

Martin Clynes
Paula Meleady
Per Bendix Jeppesen
Michael Henry
Andrew Dowd
Finbarr O'Sullivan
Lorraine O'Driscoll
Paul Dowling

Subjects

Microbiology

Topics
mass mice drug effects cell line cells secretion protein array analysis screening insulin secreting cells stress superoxide dismutase protein expression proteins protein folding metabolism insulin proteomics matrix assisted laser endoplasmic reticulum er oxidative stress pharmacology 2d dige proteome electrophoresis gel two dimensional insulin secretion isolation purification animals spectrometry subcellular fractions glucose

Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress. (2006)

Abstract The glucose-sensitive insulin-secretion (GSIS) phenotype is relatively unstable in long-term culture of beta cells. The purpose of this study was to investigate relative changes in the proteome between glucose-responsive (low passage) and glucose non-responsive (high passage) murine MIN-6 pancreatic beta cells. The 2D-DIGE and subsequent DeCyder analysis detected 3351 protein spots in the pH range of 4-7. Comparing MIN-6(H) to MIN-6(L) and using a threshold of 1.2-fold, the number of proteins with a decrease in expression level was 152 (4.5%), similar was 3140 (93.7%) and increased 59 (1.8%). From the differentially expressed proteins identified in this study, groups of proteins associated with the endoplasmic reticulum (ER) and proteins involved in oxidative stress were found to be significantly decreased in the high-passage (H passage) cells. These proteins included endoplasmic reticulum protein 29 (ERp29); 78-kDa glucose-related protein, (GRP78); 94-kDa glucose-related protein (GRP94); protein disulphide isomerase; carbonyl reductase 3; peroxidoxin 4 and superoxide dismutase 1. These results suggest that non-GSIS MIN-6 cells do not have the same ability/capacity of glucose-responsive MIN-6 cells to successfully fold, modify or secrete proteins and counteract the problems associated with oxidative stress.
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Full list of authors on original publication

Martin Clynes, Paula Meleady, Per Bendix Jeppesen, Michael Henry, Andrew Dowd, Finbarr O'Sullivan, Lorraine O'Driscoll, Paul Dowling

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Paula Meleady
Dublin City University
Total Publications: 95
 
3
Michael Henry
Dublin City University
Total Publications: 78
 
4
Finbarr O'Sullivan
Dublin City University
Total Publications: 21
 
5
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 152
 
6
Paul Dowling
Dublin City University
Total Publications: 79