Type

Journal Article

Authors

Martin Clynes
Sharon Glynn
Michael Henry
Andrew Dowd
Paula Meleady
Paul Dowling

Subjects

Biochemistry

Topics
female carcinoma ductal breast chemistry genetics proteomics mass pathology gene expression profiling paclitaxel neoplasm invasiveness electrophoresis gel two dimensional membrane proteins spectrometry neoplasm proteins gene expression regulation neoplastic humans pharmacology cell line tumor breast neoplasms matrix assisted laser

Proteomic analysis of isolated membrane fractions from superinvasive cancer cells. (2006)

Abstract The superinvasive phenotype exhibited by paclitaxel-selected variants of an in vitro invasive clonal population of the human cancer cell line, MDA-MB-435S were examined using DIGE (Fluorescence 2-D Difference Gel Electrophoresis) and mass spectrometry. Isolation of membrane proteins from the MDA-MB-435S-F/Taxol-10p4p and parental populations was performed by temperature-dependent phase partitioning using the detergent Triton X-114. Subsequent DIGE-generated data analysed using Decyder software showed many differentially-expressed proteins in the membrane fraction. 16 proteins showing statistically significant upregulation in the superinvasive cells were identified by MALDI-ToF. Proteins upregulated in the superinvasive population include Galectin-3, Cofilin, ATP synthase beta subunit, voltage-dependent anion channel 1, voltage dependent anion channel 2, ER-60 protein, MHC class II antigen DR52, Beta actin, TOMM40 protein, Enolase 1, Prohibitin, Guanine nucleotide-binding protein, Annexin II, Heat shock 70 kDa protein, Stomatin-like protein 2 and Chaperonin. Many of these proteins are associated with inhibition of apoptosis, the progression of cancer, tumourigenicity, metastasis, actin remodelling at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility. These results suggest a correlation between the increased abundance of these proteins with the superinvasive phenotype of the paclitaxel-selected MDA-MB-435S-F/Taxol-10p4p population.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Martin Clynes, Sharon Glynn, Michael Henry, Andrew Dowd, Paula Meleady, Paul Dowling

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Sharon A Glynn
National University of Ireland Galway
Total Publications: 21
 
3
Michael Henry
Dublin City University
Total Publications: 78
 
4
Paula Meleady
Dublin City University
Total Publications: 95
 
5
Paul Dowling
Dublin City University
Total Publications: 79