Type

Journal Article

Authors

Martin Clynes
Judith H Harmey
Deirdre Cronin
Brian Gibson
Aine Adams
Sharon A Glynn

Subjects

Biochemistry

Topics
chemotaxis female mice inbred balb c animals cell movement cell adhesion mice pathology doxorubicin secondary extracellular matrix paclitaxel lung neoplasms adenocarcinoma neoplasm invasiveness cell growth processes pharmacology drug effects physiology mammary neoplasms experimental cell line tumor

Low adhesiveness coupled with high superinvasiveness in vitro predicts the in vivo metastatic potential of a mouse mammary adenocarcinoma cell line. (2006)

Abstract We have previously shown that the selection of cancer cell lines with chemotherapeutic agents can alter the invasive potential of the cells, resulting in a superinvasive phenotype, where cells not only invade through matrigel and migrate through membrane pores, but also subsequently detach from the underside of the invasion chamber, survive in suspension and ultimately attach to and grow on the bottom of the well beneath the insert. In order to determine the significance of this in vivo, the following experiments were performed. 4T1-GFP mouse mammary adenocarcinoma cells were pulse-selected with doxorubicin or paclitaxel. Three variants with differing invasiveness were isolated and their metastatic potential in vivo was compared to the parental cell line, through injection into the mammary fat pad of BALB/c mice and subsequent primary tumour growth and metastasis to the lungs measurement. Increasing superinvasiveness was inversely linked to tumour diameter (p < 0.005). The superinvasive status predicted the incidence of lung nodules in 2/3 variant groups, with significant differences in the number of nodules (p < 0.001). The in vitro superinvasive phenotype coupled with decreased adhesion may predict for metastatic potential in vivo.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Martin Clynes, Judith H Harmey, Deirdre Cronin, Brian Gibson, Aine Adams, Sharon A Glynn

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Judith H Harmey
Royal College of Surgeons in Ireland
Total Publications: 30
 
3
Sharon A Glynn
National University of Ireland Galway
Total Publications: 21