Type

Journal Article

Authors

Malcolm R. Smyth
Robert J. Forster
Richard O'Kennedy
Anthony J. Killard
Máire B. O'Connor

Subjects

Chemistry

Topics
antibodies cross reactions animals immunology ruthenium compounds chemistry osmium compounds rabbits luminescence immunoenzyme techniques

Production and characterization of a polyclonal antibody for Os(II) and Ru(II) polypyridyl complexes. (2005)

Abstract The characterization of a polyclonal antibody produced via immunization with an [Os(bpy)(2)dcbpy] hapten is described. Bpy is 2,2'-bipyridine and dcbpy is 2,2'-bipyridine-4,4'-dicarboxylic acid. The cross-reactivity of the antibody for the Ru(II) analogue of the hapten was also investigated. Large increases in the emission and luminescent lifetime of a series of Os and Ru complexes were observed on binding of the antibody. Association equilibrium constants were derived from luminescence titration data and were found to be 5.6 x 10(8) and 5.0 x 10(8)M(-1) for [Os(bpy)(2)dcbpy] and [Ru(bpy)(2)dcbpy], respectively. Spectroscopic changes were likely due to the exclusion of H(2)O from the complex/antibody binding cleft and blocking of vibrational relaxation pathways of the Os/Ru excited state. D(2)O/H(2)O experiments confirmed that the antibody protected approx. 82% of [Os(bpy)(2)dcbpy] and 80% of [Ru(bpy)(2)dcbpy] from excited state deactivation by the aqueous solvent.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Malcolm R. Smyth, Robert J. Forster, Richard O'Kennedy, Anthony J. Killard, Máire B. O'Connor

Experts in our system

1
Robert J. Forster
Dublin City University
Total Publications: 90
 
2
R O'Kennedy
Dublin City University
Total Publications: 197
 
3
Anthony J. Killard
Dublin City University
Total Publications: 73