Type

Journal Article

Authors

Per Bendix Jeppesen
Martin Clynes
Lorraine O'Driscoll
Erica Hennessy

Subjects

Biochemistry

Topics
min6 genetics glucose animals insulin secreting cells insulin mice gsis insulin secretion genes society cells secretion metabolism pharmacology humans low density drug effects microrna micrornas cell line tumor

Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells. (2010)

Abstract MicroRNAs (miRNAs) are a family of endogenous small non-coding RNAs which regulate mRNAs at the post-transcriptional level. MiRNAs have been identified in both normal physiological and pathological conditions. To date, a limited number of miRNAs have been shown to be involved in the regulation of insulin secretion. We have identified a panel of 10 miRNAs down-regulated in glucose non-responsive MIN6 cells compared to glucose responsive cells using TaqMan Low Density human microRNA arrays. Of these 10 miRNA targets, subsequent functional investigations involving knockdown of mir-200a, mir-130a and mir-410 levels suggested that they may decrease the capability of MIN6 cells to secrete insulin in response to stimulatory levels of glucose. Conversely, experiment with over-expression of mir-410 suggest that it may enhance levels of glucose stimulated insulin secretion. In this study, we have also identified 21 miRNAs not previously known to have a potential murine homolog.
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Full list of authors on original publication

Per Bendix Jeppesen, Martin Clynes, Lorraine O'Driscoll, Erica Hennessy

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 152
 
3
Erica Hennessy
Dublin City University
Total Publications: 5