Journal Article


M Clynes
J Keenan
Y Liang
R O'Connor
M Heenan
P Gammell
S A Glynn


Medicine & Nursing

drug effects drug therapy breast neoplasms cell adhesion cell movement antineoplastic combined chemotherapy protocols pathology doxorubicin administration dosage humans adult neoplasm invasiveness pharmacology paclitaxel selection genetic phenotype carcinoma ductal breast female drug resistance neoplasm

A new superinvasive in vitro phenotype induced by selection of human breast carcinoma cells with the chemotherapeutic drugs paclitaxel and doxorubicin. (2004)

Abstract Doxorubicin- and paclitaxel-selected variants of an in vitro invasive clonal population of the human breast cancer cell line, MDA-MB-435S, were established by pulse selection, and exhibited a novel 'superinvasive' phenotype. This phenotype is characterised by an ability to relocate to another surface following invasion through matrigel and membrane pores, by decreased adhesion to extracellular matrix proteins and by increased motility. This may represent an in vitro model of a step in the metastatic process occurring subsequent to invasion. The paclitaxel-resistant variants, MDA-MB-435S-F/Taxol-10p and MDA-MB-435S-F/Taxol-10p4p were resistant to paclitaxel, vincristine and docetaxel, but not to doxorubicin, carboplatin, etoposide or 5-fluorouracil. The doxorubicin-selected variants MDA-MB-435S-F/Adr-10p and MDA-MB-435S-F/Adr-10p10p, in contrast, exhibited only small increases in resistance to doxorubicin, although they were slightly resistant to VP-16 and docetaxel, and exhibited increased sensitivity to paclitaxel, carboplatin and 5-fluorouracil.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

M Clynes, J Keenan, Y Liang, R O'Connor, M Heenan, P Gammell, S A Glynn

Experts in our system

Martin Clynes
Dublin City University
Total Publications: 232
Joanne Keenan
Dublin City University
Total Publications: 27
Robert O'Connor
Dublin City University
Total Publications: 74
Sharon A Glynn
National University of Ireland Galway
Total Publications: 21