Type

Journal Article

Authors

Richard O'Kennedy
Brian Fitzpatrick

Subjects

Microbiology

Topics
antibodies antigen antibody complex immunology extracellular matrix proteins reference standards cattle chemistry surface plasmon resonance metabolism warfarin blood immunoassay ligands sensitivity and specificity reproducibility of results calibration antigens pharmacology animals substrate specificity sodium chloride hemofiltration chromatography high pressure liquid serum albumin bovine ultrafiltration drug effects humans

The development and application of a surface plasmon resonance-based inhibition immunoassay for the determination of warfarin in plasma ultrafiltrate. (2004)

Abstract Warfarin is the most widely prescribed oral anticoagulant for the management of a wide variety of thromboembolic disorders such as atrial fibrillation and deep vein thrombosis. A panel of warfarin-protein conjugates were produced and characterised and subsequently used for the production of monoclonal antibodies to warfarin. Following characterisation, the monoclonal antibodies were used in the development of a surface plasmon resonance-based inhibition immunoassay for the determination of the physiologically active 'nonprotein'-bound fraction of the drug in plasma ultrafiltrate. The inhibition immunoassay was compared with an existing high-performance liquid chromatography (HPLC) chromatographic technique for the determination of warfarin in plasma ultrafiltrate, and an excellent correlation was achieved between the two independent analytical techniques. The ligand-binding capacity and stability of various immobilised ligands were also compared. The BIACore-based inhibition immunoassay demonstrated an assay precision range of approximately 4-250 ng/ml, which is within the clinical range and demonstrated good reproducibility and robustness.
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Full list of authors on original publication

Richard O'Kennedy, Brian Fitzpatrick

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197