Type

Journal Article

Authors

Richard O'Kennedy
John Quinn
Stephen Hearty
Paul Leonard

Subjects

Microbiology

Topics
animals food microbiology immunology surface plasmon resonance listeria monocytogenes time factors immunoglobulin fab fragments rabbits goats isolation purification

A generic approach for the detection of whole Listeria monocytogenes cells in contaminated samples using surface plasmon resonance. (2003)

Abstract The opportunistic food pathogen Listeria monocytogenes is of great concern to the food industry and its rapid detection is of major importance. This paper describes the detection of L. monocytogenes with a polyclonal antibody by means of a new subtractive inhibition assay using a BIAcore 3000 biosensor. Incubating L. monocytogenes cells and antibody for a short period of time, followed by subsequent separation of free unbound antibody with a stepwise centrifugation process, allowed the detection of 1 x 10(5) L. monocytogenes cells/ml in less than 30 min. Free antibody was passed over an anti-Fab ligand-coated sensor chip surface with the generated response being inversely proportional to the inhibiting cell concentration. The method was simple, rapid and needed minimum sample preparation. This assay format has the potential for the quick and sensitive detection of pathogens with limited sample handling and preparation.
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Full list of authors on original publication

Richard O'Kennedy, John Quinn, Stephen Hearty, Paul Leonard

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Stephen Hearty
Dublin City University
Total Publications: 27
 
3
Paul Leonard
Dublin City University
Total Publications: 27