Journal Article


Craig E Lunte
Richard O'Kennedy
Malcolm R. Smyth
Eimear M Ward



animals male chemistry metabolism electrophoresis capillary coumarin haplorhini coumarins technology pharmaceutical microsomes hydrogen ion concentration

Application of capillary electrophoresis with pH-mediated sample stacking to analysis of coumarin metabolites in microsomal incubations. (2003)

Abstract A sensitive method for the analysis of metabolites of coumarin by capillary electrophoresis (CE), incorporating pH-mediated sample stacking, was developed. The analytes were detected in phosphate buffer (pH 7.5; 25 mM), the matrix of the microsomal incubations. Detection was by direct UV absorbance. The three metabolites studied were 7-hydroxycoumarin (7-OHC), 4-hydroxycoumarin (4-OHC) and 2-hydroxyphenylacetic acid (HPAA), and the limits of detection of the analytes were 0.1, 0.5 and 0.3 microM, respectively. The developed method was then applied to microsomal incubations of coumarin. Male Cynomologus monkey microsomes were used in the study and 7-OHC was detected in the incubation mixture.
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Full list of authors on original publication

Craig E Lunte, Richard O'Kennedy, Malcolm R. Smyth, Eimear M Ward

Experts in our system

R O'Kennedy
Dublin City University
Total Publications: 197