Journal Article


M Clynes
P Murphy
A M Larkin
R O'Connor
M O'Keeffe
E Moran



antigen antibody reactions animals benzimidazoles immunology haptens 5 benzimidazolecarboxylic acid enzyme linked immunosorbent assay surface plasmon resonance carrier proteins mice inbred balb c mice immunization antibodies monoclonal carboxylic acids biosynthesis

Methods for generation of monoclonal antibodies to the very small drug hapten, 5-benzimidazolecarboxylic acid. (2002)

Abstract Drug-specific monoclonal antibodies (MAbs) were produced against the very small drug hapten (162.15 Da), 5-benzimidazolecarboxylic acid, an analogue of 2-(4-Thiazolyl)benzimidazole (TBZ) but lacking the thiol group. TBZ is widely used as a broad-spectrum anthelmintic in various animal species and humans and also as a food preservative and agricultural fungicide. The anti-5-benzimidazolecarboxylic acid antibodies produced have potential use for extraction and/or detection of protein-bound residue forms of TBZ. Three in vivo immunisation regimes (with combinations of two related small drug haptens and two different adjuvants/carrier molecules) and an in vitro immunisation procedure using a combination of three related unconjugated small drug haptens were investigated. Specificity for the hapten immunogen/s was initially determined using two different ELISA procedures. BIACORE analysis, in conjunction with drug binding inhibition studies, was used to confirm the specificity of a small number of selected clones. In vivo immunisation with a drug molecule conjugated to a lipopeptide/T-cell epitope, which acts both as a carrier molecule and an adjuvant was the most useful of the methods tested for the production of specific MAbs to a typically very small hapten with low immunogenic properties.
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Full list of authors on original publication

M Clynes, P Murphy, A M Larkin, R O'Connor, M O'Keeffe, E Moran

Experts in our system

Martin Clynes
Dublin City University
Total Publications: 232
Paul V Murphy
National University of Ireland Galway
Total Publications: 73
Annemarie Larkin
Dublin City University
Total Publications: 26
Robert O'Connor
Dublin City University
Total Publications: 74
Mary O'Keeffe
University of Limerick
Total Publications: 57