Type

Journal Article

Authors

M R Morgan
H A Lee
R O'Kennedy
B M Manning
P P Dillon
G J Keating
S J Daly

Subjects

Microbiology

Topics
aflatoxin b1 reproducibility of results enzyme linked immunosorbent assay rabbits female sensitivity and specificity serum albumin bovine antibodies surface plasmon resonance animals

Development of surface plasmon resonance-based immunoassay for aflatoxin B(1). (2000)

Abstract Aflatoxins are a group of highly toxic fungal secondary metabolites that occur in Aspergillus species and may contaminate foodstuffs and feeds. Two different anti-aflatoxin B(1) antibodies were examined to develop a surface plasmon resonance (SPR)-based immunoassay to aflatoxin B(1). A conjugate consisting of aflatoxin B(1)-bovine serum albumin (BSA) was immobilized on the dextran gel surface. Competition between immobilized aflatoxin B(1) conjugate and free aflatoxin B(1) in solution for binding to antibody injected over the surface formed the basis for the assay. Regeneration of the antibody from the immobilized conjugate surface is essential for the development of such an inhibitive immunoassay. Problems were encountered with the regeneration of the sensor surface, due to the high-affinity binding of the antibodies. Conventional regeneration solutions consisting of low concentrations of NaOH and HCl worked to a degree, but regeneration was at the expense of the integrity of the immobilized conjugate. A polyclonal anti-aflatoxin B(1) antibody was produced and was found to be regenerable using an organic solution consisting of 1 M ethanolamine with 20% (v/v) acetonitrile, pH 12.0. This combined high ionic strength and extreme pH, as well as chaotrophic properties and allowed the development of an inhibitive immunoassay. The assay had a linear range of 3.0-98.0 ng mL(-1) with good reproducibility.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

M R Morgan, H A Lee, R O'Kennedy, B M Manning, P P Dillon, G J Keating, S J Daly

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197