Type

Journal Article

Authors

M Clynes
S M Kennedy
L Connolly
G Doherty
D Alexander
E Moran
A Larkin

Subjects

Biochemistry

Topics
immunohistochemistry ribonucleases antibodies monoclonal cercopithecus aethiops rats atp binding cassette transporters antigen antibody reactions genetics cell fusion multidrug resistance protein 3 animals metabolism genes mdr leukemia b cell cell line reverse transcriptase polymerase chain reaction blotting western rats inbred buf mice antibody specificity genetic code mice transgenic p glycoproteins humans

A new monoclonal antibody that specifically recognises the MDR-3-encoded gene product. (1999)

Abstract The MDR-3-encoded P-glycoprotein (Pgp) is highly expressed in liver and is thought to function as a hepatic transporter of phospholipids into bile. However its role, if any, in other tissues remains undefined. Although transfection experiments have indicated that it may be unable to confer drug resistance, there is evidence that it may be involved in drug resistance in certain B-cell leukaemias. To date, most work on clinical samples has been performed at the mRNA level; limited work has been performed using polyclonal antibodies raised to MDR-3 and mdr-2 (the murine equivalent of MDR-3). We have generated a new monoclonal antibody, termed 6/1G, which specifically recognises the human MDR-3 gene-encoded product. Antibody 6/1G was produced by in vitro immunisation of spleen cells from BALB/c mice with a synthetic 12-amino acid peptide. Cells from MDR-3 transgenic mice showed consistent membranous staining with antibody 6/1G. Immunoblotting with 6/1G identified a band at 170 kDa on lysates of MDR-3 transgenic cells. Preliminary results with a range of B-cell leukaemias suggest that MDR-3 Pgp positivity may be a marker for a more malignant phenotype in B-CLL. Antibody 6/1G may be useful in defining a role for MDR-3 in malignancy and drug resistance, as well as in certain liver diseases such as progressive familial intracholeostasis.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

M Clynes, S M Kennedy, L Connolly, G Doherty, D Alexander, E Moran, A Larkin

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Susan Kennedy
Dublin City University
Total Publications: 37
 
3
Annemarie Larkin
Dublin City University
Total Publications: 25