Type

Journal Article

Authors

R O'Kennedy
M R Smyth
E I Iwuoha
B Lu

Subjects

Microbiology

Topics
antigen antibody reactions chemistry immunology horseradish peroxidase solvents acetonitriles acetonitrile horseradish peroxidase hrp

Effects of acetonitrile on horseradish peroxidase (HRP)-anti HRP antibody interaction. (1997)

Abstract The effects of the water-miscible organic solvent acetonitrile on the enzymatic activity of horseradish peroxidase (HRP) and on HRP-anti-HRP binding have been investigated. Results showed that both the catalytic activity of HRP and the binding ability of the antibody were affected on increasing the concentration of the organic solvent. The activity of HRP varied with the organic composition of the solvent, indicating that the conformation of the enzyme was affected. The binding ability of the antibody also decreased significantly with an increase of the organic composition of the solvent, and in absolute acetonitrile, the activity of the antibody is about 500 times lower than that in aqueous medium. Binding reversibility experiments indicated that the antibody was not irreversibly damaged in solutions with acetonitrile composition greater than 80% and below 40%; however, an irreversible decrease in the binding was observed in solutions with an acetonitrile composition between 40 and 80%. The reduction in the binding ability is probably due to the irreversible conformation changes in the antibody.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

R O'Kennedy, M R Smyth, E I Iwuoha, B Lu

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
E I Iwuoha
Dublin City University
Total Publications: 10