Type

Journal Article

Authors

J Wang
R O'Kennedy
D Egan
M R Smyth
C O'Sullivan
E Dempsey

Subjects

Microbiology

Topics
7 hydroxycoumarin umbelliferones antibody specificity kinetics chemistry immunology hydrogen ion concentration electrochemistry antibodies electrodes biosensing techniques

Development of an antibody-based amperometric biosensor to study the reaction of 7-hydroxycoumarin with its specific antibody. (1993)

Abstract An amperometric biosensor, based on an anti-7-hydroxycoumarin (7-OH-coumarin) antibody immobilized at the surface of a glassy carbon electrode, and contained behind a cellulose dialysis membrane, is described. The electrochemical behaviour of this metabolite at the bare glassy carbon electrode was found to be well defined by using differential-pulse voltammetry, and a 90% decrease in peak height was observed on binding of the antibody to the antigen, which occurs at the electroactive site of 7-OH-coumarin. The kinetics of the antibody-antigen reaction was investigated and a first-order reaction was observed with k = 0.0329 mA min-1. This system provides a novel method for studying antibody specificity and the kinetics of such antibody-antigen interactions.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

J Wang, R O'Kennedy, D Egan, M R Smyth, C O'Sullivan, E Dempsey

Experts in our system

1
Jun Wang
Trinity College Dublin
Total Publications: 19
 
2
R O'Kennedy
Dublin City University
Total Publications: 197
 
3
Denise A Egan
IT Tallaght
Total Publications: 44
 
4
Eithne Dempsey
IT Tallaght
Total Publications: 17