Type

Journal Article

Authors

M Clynes
E Moran
A Redmond

Subjects

Microbiology

Topics
therapeutic use humans drug resistance p glycoprotein drug effects teniposide ovarian neoplasms etoposide carrier proteins membrane glycoproteins female chemistry tumor cells cultured genetics oligonucleotides antisense transfection doxorubicin vincristine drug therapy

Multiple drug resistance in the human ovarian carcinoma cell line OAW42-A. (1993)

Abstract A new multidrug-resistant variant (OAW42-A) of a human ovarian carcinoma line has been selected by exposure to increasing concentrations of doxorubicin. The variant is resistant to doxorubicin, vincristine (but surprisingly not to colchicine), etoposide, tenoposide and also to cisplatin (a drug not usually involved in classical multidrug resistance), but not to 5-fluorouracil. Overexpression of P-glycoprotein in the resistant line was demonstrated by immunofluorescence and western blotting. Direct evidence for P-glycoprotein as a determinant of resistance was provided by transfection with a specific antisense oligonucleotide. Reversal was incomplete and this, along with the pattern of cross-resistance observed, suggests that additional mechanisms of resistance may also be involved. Substantial clonal variation in resistance exists within the cell line.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

M Clynes, E Moran, A Redmond

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
A Redmond
IT Tallaght
Total Publications: 8