Type

Journal Article

Authors

R O'Kennedy
O Nolan

Subjects

Microbiology

Topics
humans drug design binding sites antibody t lymphocytes animals immunology antibodies monoclonal drug carriers therapeutic use biosynthesis

Bifunctional antibodies: concept, production and applications. (1990)

Abstract Immunoglobulins, or antibodies, are monospecific, bivalent antigen-binding molecules. Bifunctional antibodies are bispecific, with each arm binding to a different antigen, and may be produced by biological or chemical methods. Biological production involves the fusion of two monoclonal antibody-producing hybridomas or of an immunised spleen cell and a hybridoma. The resulting hybrid hybridomas (quadromas or triomas) secrete a mixture of parenteral monoclonal antibodies and bifunctional antibody. In chemical production, the parental monoclonal antibodies can be 'chopped up and reconstituted' to produce the bifunctional antibody only. Bifunctional antibodies have a variety of potential uses. They were originally proposed as an aid to cancer chemotherapy where one of the arms of the antibody would bind to a tumour marker and the other to a drug, toxin, or cytotoxic cell. Functional agents can thus be target directly onto tumour cells, accumulating with higher density, yet with reduced side effects for the patient. Further applications have been proposed involving enzyme immobilization and novel immunoassay techniques. This review describes developments that have taken place in bifunctional antibody technology to date.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

R O'Kennedy, O Nolan

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197