Type

Journal Article

Authors

M R Smyth
C G Kilty
R O'Kennedy
C O'Fágáin
J M Fernandez Alvarez

Subjects

Chemistry

Topics
alanine transaminase differential pulse voltammetry enzyme stability electrochemistry humans cyclic voltammetry spectrophotometry ultraviolet

Application of adsorptive voltammetry to assess the stability of modified alanine aminotransferases. (1990)

Abstract Alanine aminotransferase has been stabilized by using chemical modification with both bis(imidates) (of varying length) and succinic anhydride. The voltammetric behavior of the native enzyme and its various modified forms has been studied by using both cyclic voltammetry and differential pulse adsorptive voltammetry. A distinctive accumulation pattern was found for each of the stabilized enzymes at the static mercury drop electrode with respect to the native alanine aminotransferase. Adsorptive voltammetry was demonstrated to be a useful technique to assess the extent of chemical modification of this enzyme, which is indirectly related to their stability for use in biotechnological processes. The sue of differential pulse adsorptive voltammetry, after a preconcentration of the enzyme for 300 s at the electrode surface, has yielded a detection limit of 1.0 x 10(-9) M.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

M R Smyth, C G Kilty, R O'Kennedy, C O'Fágáin, J M Fernandez Alvarez

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197