Type

Journal Article

Authors

Fergal J O'Brien
Thomas C Flanagan
Stefan Jockenhoevel
Tanya J Levingstone
Claire M Brougham

Subjects

Engineering

Topics
human cells heart valve biomaterials collagen mechanical testing cell matrix molecular cellular and tissue engineering tissue engineering mechanical properties biomedical engineering and bioengineering structural biology biology and biomimetic materials fibrin cardiovascular scaffold collagen glycosaminoglycan scaffold smooth muscle cells

Incorporation of fibrin into a collagen-glycosaminoglycan matrix results in a scaffold with improved mechanical properties and enhanced capacity to resist cell-mediated contraction. (2015)

Abstract Fibrin has many uses as a tissue engineering scaffold, however many in vivo studies have shown a reduction in function resulting from the susceptibility of fibrin to cell-mediated contraction. The overall aim of the present study was to develop and characterise a reinforced natural scaffold using fibrin, collagen and glycosaminoglycan (FCG), and to examine the cell-mediated contraction of this scaffold in comparison to fibrin gels. Through the use of an injection loading technique, a homogenous FCG scaffold was developed. Mechanical testing showed a sixfold increase in compressive modulus and a thirtyfold increase in tensile modulus of fibrin when reinforced with a collagen-glycosaminoglycan backbone structure. Human vascular smooth muscle cells (vSMCs) were successfully incorporated into the FCG scaffold and demonstrated excellent viability over 7days, while proliferation of these cells also increased significantly. VSMCs were seeded into both FCG and fibrin-only gels at the same seeding density for 7days and while FCG scaffolds did not demonstrate a reduction in size, fibrin-only gels contracted to 10% of their original diameter. The FCG scaffold, which is composed of natural biomaterials, shows potential for use in applications where dimensional stability is crucial to the functionality of the tissue. Fibrin is a versatile scaffold for tissue engineering applications, but its weak mechanical properties leave it susceptible to cell-mediated contraction, meaning the dimensions of the fibrin construct will change over time. We have reinforced fibrin with a collagen glycosaminoglycan matrix and characterised the mechanical properties and bioactivity of the reinforced fibrin (FCG). This is the first scaffold manufactured from all naturally derived materials that resists cell-mediated contraction. In fact, over 7days, the FCG scaffold fully resisted cell-mediated contraction of vascular smooth muscle cells. This FCG scaffold has many potential applications where natural scaffold materials can encourage regeneration.
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Full list of authors on original publication

Fergal J O'Brien, Thomas C Flanagan, Stefan Jockenhoevel, Tanya J Levingstone, Claire M Brougham

Experts in our system

1
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
 
2
Thomas C Flanagan
Dublin Institute of Technology
Total Publications: 5
 
3
Stefan Jockenhoevel
Dublin Institute of Technology
Total Publications: 6
 
4
Tanya J Levingstone
Royal College of Surgeons in Ireland
Total Publications: 28
 
5
Claire M Brougham
Dublin Institute of Technology
Total Publications: 9